| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Cardington-Lincoln | A | Wodecki | Denny | Goers | Higgins | NT |
| 3 | Clear Fork | A | Myers | Sansom | Eicher | Vidonish | DNS |
| 4 | Northmor | A | Ciballi | Davis | Wardle | Carson | 1:00.91 |
| 5 | Mount Gilead | A | Cross | Floyd | Dudgeon | Hoffmaster | NT |
| 6 | Cal. River Valley MS | A | Blevins | Johnson | Akers | Crabtree | NT |