| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Clear Fork | A | Weemhoff | Bowers | Kaylor | Gess | DNS |
| 3 | Cal. River Valley MS | A | Moran | Schertzer | Barnes | Burkes | NT |
| 4 | Northmor | A | Burgess | Kohls | Smith | Hanes | 5:29.30 |
| 5 | Cardington-Lincoln | A | Rammelsburg | Moodispaugh | Osborne | Long | NT |
| 6 | Mount Gilead | A | Floyd | Combs | Hoffmaster | Sergent | NT |