| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Mans. Ontario | A | Kilgore | Wagoner | Witherspoon | Van Harlingen | NT |
| 2 | Clear Fork | A | Myers | Bowers | Eicher | Roberts | NT |
| 3 | Fredericktown | A | Cockrell | Kanavel | Smith | Tenney | 59.69 |
| 4 | Northmor | A | Ciballi | Davis | Wardle | Carson | 1:00.91 |
| 5 | Lexington | A | Harwell | Dunn | Telford | Hess | NT |
| 6 | East Knox | A | Lilly | Lilly | Fratangelo | Shelley | NT |