| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clear Fork | B | Mills | Wine | Hamilton | Myers | NT | |
| 2 | Fredericktown | B | Meade | Levering | Conkle | Kelly | NT | |
| 3 | Clear Fork | A | Kime | Wade | Peteri | Swendal | 45.08 | |
| 4 | Fredericktown | A | Dickerson | Bryant | Kanavel | O'Brian | 46.14 | |
| 5 | Marion Harding | A | Estrada | Grooms | Velez | Simon | 46.65 | |
| 6 | Marion Harding | B | Banks | Blanton | Muhammad | Deeren | DNS |