| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Marion Grant | A | Santiago-Silva | Rice | Kinsey | Smith | 1:56.77 | |
| 3 | Pleasant | A | Meyers | Noland | Kelder | Cornell | NT | |
| 4 | Cal. River Valley MS | A | Fetter | Poulos | Osborne | Bell | 1:57.11 | |
| 5 | Upper Sandusky | A | England | McWilliams | Solly | Morris | NT | |
| 6 | Wynford | A | Bess | Hay | Miguel-Roblero | Grady | NT | |
| 7 | Pleasant | B | Joseph | Holman | Salinas | Webster | NT | |
| 8 | Fredericktown | A | Hoeflich | Short | Ebersole | Hansen | 2:07.14 |