| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Spr. Shawnee | A | Calhoun | Reed | DeSantis | Zettler | 56.03 | |
| 5 | West Jefferson | A | Mazza | Priest | Peters | Ater | 56.61 | |
| 6 | Spr. Northwestern | A | Thomas | Clark | King | Hensley | 57.42 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Stivers School For The Arts | A | Morgan II | Shelton | Adkins | Beasley | 54.32 | |
| 3 | Mad. Plains | A | Kingery | Vallery | Zerkle | McComas | 53.95 | |
| 4 | Kenton Ridge | A | Day | Shaffer | Rhodes | Price | 48.94 | |
| 5 | Bellefontaine | A | Fleming | Parmer | Hudson | Robb | 52.25 | |
| 6 | WCH Washington | A | Vernon | Edwards | Marken | Bennett | 53.21 | |
| 7 | Spr. Shawnee | B | Williams | Pennington | Haydon | Hoskins | 56.03 |