| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 6 | RAMMELSBURG, Avery | Cardington-Lincoln | 2:50.28 | |
| 7 | DOUP, Hazel | Danville | 2:56.02 | |
| 8 | ROBINSON, Trinity | Cardington-Lincoln | 3:03.27 | |
| 9 | BRUFF, Brooklyn | Danville | 3:07.52 | |
| 10 | RAMOS-RIOS, Nahtali | Danville | 3:08.82 | |
| 11 | PALMER, Ayla | Fredericktown | 3:20.54 | |
| 12 | HOLLAR, Adalie | Fredericktown | 3:23.49 | |
| 13 | REDMAN, Channing | Cardington-Lincoln | 3:33.01 | |
| 14 | PAYNE, Isabelle | Danville | 3:48.02 |