| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Upper Sandusky | A | Barr | Pelfrey | Klaus | Alspach | 47.64 | |
| 3 | St. Paul | A | Couch | Pocos | Caizzo | Houser | 47.30 | |
| 4 | Bath | A | Mikesell | Moreland | Walls | Mayer | 45.03 | |
| 5 | Fredericktown | A | Sipes | Toombs | Roush | Estep | 45.04 | |
| 6 | Mohawk | A | Stansbery | Ekleberry | Coffman | Schimpf | 45.71 | |
| 7 | Cal. River Valley | A | Davis | Smith | Dodds | Babb | 50.84 |