| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Upper Sandusky | A | Barr | Smith | Pelfrey | Stringfellow | 1:41.30 | |
| 2 | St. Paul | A | Springer | Alt | Houser | Pocos | 1:37.70 | |
| 3 | Bath | A | Mikesell | Moreland | Walls | Mayer | 1:37.08 | |
| 4 | Bellefontaine | A | Jones | Sechrest | Butler III | Foulk | 1:32.08 | |
| 5 | Fredericktown | A | Rine | Garver | Estep | Sipes | 1:35.05 | |
| 6 | Mohawk | A | Schimpf | Ekleberry | Coffman | Stansbery | 1:36.90 | |
| 7 | Cal. River Valley | A | Hummel | Collins | Lee | Burns | 1:39.16 |