| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clear Fork | C | Miller | Mills | Baughman | Carroll | NT | |
| 2 | Mar. Highland | A | Ballard | Ramey | Kanagy | Bowers | 1:06.86 | |
| 3 | Clear Fork | A | Thrush | Freeman | Cyphers | Finley | 51.79 | |
| 4 | Galion | A | Jackson | Drake | Chambers | Kessler | 56.36 | |
| 5 | Clear Fork | B | Tackett | Miller | Donelson | Briggs | NT |