| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fredericktown | B | McGuckin | Budd | Bostic | Dickerson | 3:55.43 | |
| 3 | Cardington-Lincoln | A | Compton | Patterson | McNichols | Abuayyash | 3:48.62 | |
| 4 | Fredericktown | A | Garver | Marilo | Hartley | Wenger | 3:42.71 | |
| 5 | Northmor | A | Hunter | Snyder | Bower | Dean | 3:43.49 | |
| 6 | Clear Fork | A | Bostic | Bunnell | Sullivan | Peteri | 3:43.86 | |
| 7 | Be Panthers | A | McCormick | Bossard | Workman | Bossard | DNS |