| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Johnstown | A | Kisner | Sisson | Swick | Hampton | NT | |
| 2 | East Knox | A | Fratangelo | Shepard | Flowers | Howard | 5:26.08 | |
| 3 | Mount Gilead ms | A | Ferrell | Floyd | Hoffmaster | Combs | 5:12.07 | |
| 4 | Utica | A | McKinney | Pryor | Shaver | McInturff | 4:56.40 | |
| 5 | Columbus School for Girls | A | Howell | Coburn | Sorce | Seals | 4:57.80 | |
| 6 | Mar. Highland | A | Jackson | Rhea | Blanchard | Trainer | 5:01.46 | |
| 7 | Fredericktown | A | Levering-Smith | Sheriff | Mckean | Platt | 5:30.43 |