| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fredericktown | A | Carpenter | Short | Tier | Timmons | 4:51.33 | |
| 3 | East Knox | A | Faucett | Williams | Fields | Arnold | 4:34.76 | |
| 4 | Mar. Highland | A | Lightle | Sines | Westhoven | Eldred | 4:06.03 | |
| 5 | Mount Gilead ms | A | Keever | Grimm | Sayers | Williams | 4:11.71 | |
| 6 | Utica | A | Ulrich | Green | Meek | Dodd | 4:31.85 |