| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Utica | A | Dunn | Pryor | Morey | Heckman | NT | |
| 2 | Mount Gilead ms | A | Bowie | Nettle | Rizor | Douglas | 2:10.12 | |
| 3 | Mar. Highland | A | Schirtzinger | Bowersmith | Williams | Moore | 2:05.71 | |
| 4 | Johnstown | A | Thompson | Heimerl | Kisner | Slack | 2:02.64 | |
| 5 | Fredericktown | A | Smith | D'Andrea | McGuckin | Cockrell | 2:04.66 | |
| 6 | Columbus School for Girls | A | Moore-Utley | Abdi | Feher | McVey | 2:05.14 | |
| 7 | East Knox | A | Ferguson | Lilly | Kelley | Shelley | 2:10.97 |