| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fredericktown | B | Gearheart | Conkle | Levering | Kelly | 1:04.63 | |
| 3 | Danville | A | Gronberg | Joseph | King | Sampson | 51.13 | |
| 4 | Centerburg | A | Ball | Joiner | Indiciani | Love | 44.65 | |
| 5 | Fredericktown | A | Dickerson | Bryant | Kanavel | O'Brian | DNS | |
| 6 | Centerburg | B | Waggoner | Clayton | Cornejo | Crane | 46.58 | |
| 7 | Centerburg | C | Tiffany | Hayden | Andrews | Thompson | NT |