| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Elgin | A | Conner | Leonard | Schnipke | Gear | 55.76 | |
| 4 | Marion Harding | A | Yancey | Parker | Brownridge | Campbell | 53.04 | |
| 5 | Cal. River Valley | A | Facione | Gilliam | Beechum | Strine | 54.09 | |
| 6 | Pleasant | A | Squires | Ream | Campbell | Corwin | 54.50 |