| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCRAE, Marlena | Mans. Christian | 1:00.89 | |
| 2 | CASE, Leah | Lucas | 57.62 | |
| 3 | SONGER, Faith | Crestline | 56.47 | |
| 4 | TRUMPOWER, Riley | Lucas | 48.66 | |
| 5 | HILL, Addison | Crestline | 53.13 | |
| 6 | HILL, Ahzirrea | St. Peter's | 54.89 | |
| 7 | RICKER, Natalie | Mans. Christian | 59.51 |