| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Crestline | A | Kelly | Songer | Belcik | Seymour | 1:02.19 | |
| 4 | Lucas | A | Shay | Strassell | Cuthbertson | Midkiff | 54.96 | |
| 5 | Mans. Christian | A | Defrancisco | Ricker | Roggio | Neal | 55.51 | |
| 6 | St. Peter's | A | Barry | McCrary | Powell | Hill | 59.72 |