| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lucas | A | Kendall | Rice | Fairchild | Green | NT | |
| 3 | Cardington-Lincoln | A | Levering | Dudgeon | Marquise | Townsend | NT | |
| 4 | Ridgemont | A | Nichols | Moore | Howard | Kiser | DQ | |
| 5 | Northmor | A | Creswell | Knight | Pope | Payne | 58.90 | |
| 6 | Mount Gilead | A | Newson | Long | Bulkowski | McCarty | 1:00.70 | |
| 7 | Highland | A | Caldwell | Nauman | Walker | Osborne | NT | |
| 8 | Pleasant | A | Bailey | Osborne | Mattix | Yancey | NT |