| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Highland | A | Morgan | Barnett | Hinkle | Laracuente | 2:01.00 | |
| 3 | Lucas | A | Brown | Walter | Case | Chamberlain | 2:00.00 | |
| 4 | Mount Gilead | A | Shipman | Jones | White | Elson | 1:56.00 | |
| 5 | Pleasant | A | Aurigemma | Obenour | Cobb | Pratt | 1:59.00 | |
| 6 | Cardington-Lincoln | A | Jolliff | Whetnall | Greenawalt | Meade | 2:00.00 | |
| 7 | Northmor | A | Conant | Headley | Miller | York | 2:05.00 | |
| 8 | Elgin | A | Girton | Waldo | Seiter | Mathews | 2:10.00 |