| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mount Vernon | B | Lacksonen | Conant | Pearce | Waggoner | NT | |
| 3 | Fredericktown | A | Fernandez | Woodring | Overholt | Grennell | NT | |
| 4 | Mount Vernon | A | Craigo | Foster | Young | Dowell | 45.93 | |
| 5 | West Holmes | A | Cornwell | Dye | Zollars | Williams-Dixon | 47.12 | |
| 6 | Mount Vernon | C | Baker | White | Mundy | Mullins | NT | |
| 7 | Mount Vernon | D | Parsole | Sant | Kanuckel | Thompson | NT |