| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Fredericktown | B | Carpenter | Gladman | Baker | Scott | 2:10.00 | |
| 4 | Fredericktown | A | Feeney | Bouton | Scott | Fisher | 1:55.00 | |
| 5 | East Knox | A | Todd | Campos | Frost | Totten | 2:01.00 | |
| 6 | Centerburg | A | Dickhof | Indiciani | Tucker | Griffith | 2:03.33 |