| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fisher Catholic | A | Ellis | Bethel | Craaybeek | Hampshire | 58.21 | |
| 3 | Newark Catholic | A | Myers | Green | Capretta | Layton | 55.44 | |
| 4 | Northridge | A | Siegenthaler | Evans | Smith | Patti | 52.49 | |
| 5 | Licking Heights | A | Jaf-ogunlayi | Thompson | Vance | McDougall | 53.34 | |
| 6 | Heath | A | Wiley | Hopkins | McLaughlin | Dunlap | 55.13 | |
| 7 | Utica | A | Smith | Epps | Sanderell | Boggs | 59.32 | |
| 8 | Lakewood (Hebron) | A | Parkison | Howell | Lynch | Graham | 1:02.22 |