| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Northmor | A | Ruhl | Abrams | Keirns | James | 5:26.00 | |
| 4 | East Knox | A | Fawcett | Tucker | Streby | Bostic | 4:28.00 | |
| 5 | Fredericktown | A | Bellman | Blakesley | Overholt | Phillips | 4:50.95 | |
| 6 | Mount Gilead | A | Trainer | Pohlkotte | Neal | Rigdon | 5:11.41 |