| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Centerburg | A | Paul | Fields | Griffith | Joering | 56.05 | |
| 3 | Cardington-Lincoln | A | Jolliff | Greenawalt | Crone | Holt | 54.29 | |
| 4 | Mount Gilead | A | Shipman | Jones | White | Elson | 52.17 | |
| 5 | Fredericktown | A | Feeney | Bouton | Fisher | Cockrell | 53.11 | |
| 6 | Danville | A | French | Vasquez | Hess | Boeshart | 54.29 | |
| 7 | Northmor | A | Barler | Headley | Jones | Statler | 59.00 | |
| 8 | East Knox | A | Todd | Campos | Frost | Totten | 59.31 |