| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fredericktown | A | Scott | Wilson | Sanders | Hoam | 4:40.00 | |
| 3 | Northmor | A | Brinkman | Yunker | Ervin | Dennison | 4:40.00 | |
| 4 | Cardington-Lincoln | A | Jolliff | Hallabrin | Crone | Holt | 4:27.90 | |
| 5 | Centerburg | A | Tucker | Dickhof | Indiciani | Paul | 4:29.59 | |
| 6 | East Knox | A | Magers | Clutter | Todd | Severt | 4:34.23 | |
| 7 | Mount Gilead | A | Long | Fraizer | Staley | Pfeifer | 4:45.00 | |
| 8 | Danville | A | Vasquez | French | Hess | Workman | 5:13.44 |