| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | DOMINGUEZ HERNANDEZ, Nicole | Elgin | 1:10.00 | |
| 5 | GREEN, Aryanna | Gilead Christian | NT | |
| 6 | DEZURIK, Eva | Gilead Christian | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PRUETT, Abbie | Highland | 56.65 | |
| 3 | ARNETTE-TOMASEK, Isabelle | Highland | 55.50 | |
| 4 | PRATT, Olivia | Pleasant | 53.00 | |
| 5 | BRINKMAN, Ryann | Northmor | 55.00 | |
| 6 | OBENOUR, Tayden | Pleasant | 55.00 | |
| 7 | CASTLE, Layla | Northmor | 59.00 |