| % | Meet Record | 51.90 | Miller, Powell, Jamerson, Mixon | Bishop Hartley | 1999 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Gilead Christian | A | Green | DeZurik | Bertram | DeZurik | NT | |
| 2 | Elgin | A | Boles | Doss | St. Germain | Leonard | 58.00 | |
| 3 | South Central | A | Beverly | Fry | Smith | Schumacher | 55.80 | |
| 4 | Fredericktown | A | Scott | Enzor | Fisher | Cockrell | 53.70 | |
| 5 | Cristo Rey | A | Mancia Rodriguez | Thompson | Black | Seydy | 55.00 | |
| 6 | Bucyrus | A | Sheets | Burling | Green | Tyrrell | 55.00 | |
| 7 | Northmor | A | Conant | Jones | Sheriff | Statler | 59.00 | |
| 8 | Ridgedale | A | Jones | Brown | Staton | Murphy | 1:26.00 |