| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MOSHER, Sophie | Mount Gilead MS | 2:57.57 | Verified |
| 2 | HERSHNER, Gwen | Mount Gilead MS | 2:50.77 | Verified |
| 3 | ZEHE, Ava | Ontario MS | 3:03.45 | Unverified: OMS Dual v. Lexington |
| 4 | SHADE, Madi | Fredericktown MS | 2:59.45 | Verified |
| 5 | AMBROSE, Hannah | Galion Northmor JH | 3:14.77 | Unverified: |
| 6 | REDMOND, Gabriella | Fredericktown MS | NT | Unverified: |
| 7 | POPE, Kaylee | Galion Northmor JH | 3:12.81 | Unverified: |
| 8 | STOVER, Quinn | Clear Fork | 2:43.10 | Verified |
| 9 | KNUCKLES, McKenley | Clear Fork | 3:15.00 | Unverified: |
| 10 | O'BRIEN, Marley | Clear Fork | 3:18.11 | Verified |
| 11 | PELPHREY, Kamryn | Ontario MS | NT | Unverified: |
| 12 | WELCH, Raegan | Galion Northmor JH | NT | Unverified: |
| 13 | THEM, Ella | Ontario MS | NT | Unverified: |
| 14 | DILLON, Kai | Mount Gilead MS | 3:08.00 | Unverified: |