| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | SHEPPARD, Tiara | Centerburg | 34.00 | |
| 5 | PARKER, Skylar | Utica | NT | |
| 6 | BAKER, Callie | Utica | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | NEIGHBORGALL, Grace | Centerburg | 31.00 | |
| 3 | MOORE, Allison | Centerburg | 31.00 | |
| 4 | STOWE, Evie | Centerburg | 28.27 | |
| 5 | GRIFFITH, Olivia | Centerburg | 29.80 | |
| 6 | TUCKER, Payton | Centerburg | 30.00 | |
| 7 | FIELDS, Holly | Centerburg | 33.00 |