| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Coshocton | A | Johns | Jay | Whiteus | Rice | NT | |
| 3 | Danville | A | Lucas | Loyd | Hackman | Boeshart | 46.55 | |
| 4 | East Knox | A | Lamneck | Fawcett | Madden | Hanners | 46.90 | |
| 5 | Fisher Catholic | A | Welsh | Lee | Junge | Hoch | 53.50 | |
| 6 | Ridgedale | A | Murphy | Fosnaugh | Slaughterback | Gleespen | NT |