| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mount Gilead MS | A | Davis | Long | Bulkowski | Sayers | 4:13.68 | Verified |
| 3 | Fredericktown MS | A | Garver | Rogers | Lefevre | Overholt | 4:11.55 | Verified |
| 4 | Mar. Highland | A | Clements | Weiss | Schmidt | Nauman | 3:34.38 | Verified |
| 5 | Utica | A | Stafford | Krueger | Gbedje | Bebout | 3:49.00 | Unverified: Hank Smith Invitational - Heath |
| 6 | Howard East Knox JH | A | Boyd | Holland | Korosec | Clark | 4:06.48 | Verified |
| 7 | Johnstown-Monroe MS | A | Herb | Paugh | Brisolla | Smith | 4:40.87 | Unverified: Licking Valley Middle School Inv |