| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BROKAW, Alyson | Clear Fork | 3:00.28 | Verified |
| 2 | SANCHEZ, Larissa | Danville | 4:02.62 | Verified |
| 3 | BLUBAUGH, Claire | Clear Fork | 2:48.59 | Verified |
| 4 | SLAUGHTER, Hadley | Clear Fork | 3:22.14 | Verified |
| 5 | RUSH, Lucy | Mount Vernon | NT | |
| 6 | COUCH, Taylor | Mount Vernon | 2:57.78 | Verified |
| 7 | CLUTTER, Adrian | East Knox | 2:40.76 | Verified |
| 8 | COUCH, Alex | Mount Vernon | 2:34.26 | Unverified: Fredericktown HS Invitational |