| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | MEAD, Maren | Centerburg | 26.34 | Verified |
| 3 | BALL, Blane | Centerburg | 25.94 | Verified |
| 4 | ROBINSON, Trysten | Clear Fork | 24.58 | Verified |
| 5 | STEPHENS, Nate | Danville | 24.60 | Unverified: EK Tri |
| 6 | HANNERS, Gabe | East Knox | 25.25 | Verified |
| 7 | BURGER, Trevor | Danville | 26.50 | Unverified: |
| 8 | GRENNELL, James | East Knox | 26.53 | Unverified: |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | STEENSLAND, Morgan | Clear Fork | 24.58 | Verified |
| 2 | WILSON, Cole | Mount Vernon | 23.87 | Unverified: |
| 3 | RICHARDS, Mason | Mount Vernon | 23.52 | Verified |
| 4 | STUPKA, Joe | Clear Fork | 21.86 | Verified |
| 5 | YOUNG, Ethan | Mount Vernon | 23.42 | Unverified: |
| 6 | BAKER, Hayden | East Knox | 23.47 | Unverified: John McDonough Invitational |
| 7 | TURSKE, Mason | Centerburg | 23.91 | Verified |
| 8 | LOYD, Jackson | Danville | 23.96 | Verified |