| % | Meet Record | 43.96 | Highland | Highland |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fredericktown | A | Small | Gallogly | Bellman | Hoeflich | 47.50 | |
| 3 | East Knox | A | Lamneck | Elliott | Hanners | Madden | 47.35 | |
| 4 | Mount Gilead | A | High | Harris | Bland | High | 45.50 | |
| 5 | Centerburg | A | Ball | Mead | White | Turske | 46.00 | |
| 6 | Danville | A | Lucas | Lloyd | Hackman | Boeshart | 46.20 | |
| 7 | Northmor | A | Landin | Amens | Stockdale | Becker | 47.50 | |
| 8 | Cardington-Lincoln | A | Stover | Dudgeon | Townsend | Levering | 52.00 |