| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mans. Christian | A | 50.12 | Verified | ||||
| 3 | St. Peters | A | Bodnar | Hart | Lindsay | Wendt | 48.10 | Verified |
| 4 | Crestline | A | Jones | Perry | Schneider | Bowman | 46.88 | Verified |
| 5 | Loudonville | A | Taylor | Finley | Brecheisen | Hershberger | 48.00 | Unverified: |
| 6 | Lucas | A | Grose | Shaffer | Estep | Diehl | 48.69 | Verified |