| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mans. Christian | A | 1:00.98 | Verified | ||||
| 3 | Crestline | A | Higgins | Lavene | BAKER | Engler | 58.40 | Verified |
| 4 | Loudonville | A | Cutlip | Vermilya | Templeman | Polen | 51.00 | Unverified: |
| 5 | Lucas | A | Trumpower | Norris | Restelli | Stone | 58.01 | Verified |
| 6 | St. Peters | A | Bond | Hill | Trammell | Belmer | 59.48 | Verified |