| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BAUER, Jacob | Ohio Wesleyan |
23.45 | Denison Invitational |
| 3 | MATHEWS, Isaiah | Waynesburg |
22.37 | PAC Indoor Track & Field Championships |
| 4 | THEOBALD, Trey | Ohio Wesleyan |
23.14 | DIII All-Ohio Outdoor Championships |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | SENAT, Gregory | Ohio Wesleyan |
24.39 | Wittenberg Tiger Opener |
| 3 | BERROTT, Zach | Waynesburg |
23.62 | PAC Indoor Track & Field Championships |
| 4 | DEBAUCHE, Dequan | Waynesburg |
24.30 | PAC Track & Field Championships |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | BEST, Ethan | Waynesburg |
24.60 | 2023 YSU Indoor Track and Field Icebreaker |
| 4 | DAVIS, Ray | Waynesburg |
24.91 | Bob Shannon Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HERBERT, Kai | Waynesburg |
NT | |
| 3 | PYLES, John | Unattached | 25.00 | |
| 4 | CASELLA, Kyle | Waynesburg |
NT |