| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HENRY, Alyssa | Ohio Wesleyan |
2:38.00 | |
| 2 | DELONG, Annika | Ohio Wesleyan |
2:38.00 | |
| 3 | MUNDORFF, Jaylene | Waynesburg |
2:50.70 | Bob Shannon Invitational |
| 4 | BUTCHER, Ashley | Miami University Running Club | NT | |
| 5 | BROWN, Sydney | Denison |
NT | |
| 6 | MAIO, Angela | Otterbein |
2:34.00 | |
| 7 | SHERBAN, Emma | Miami University Running Club | NT |