| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Harvest Preparatory | A | Nichols | Broom | Brown | Bryant | NT | Unverified: |
| 3 | New Hope Christian Academy | A | Pohlman | Parker | Cook | Hamilton | NT | Unverified: |
| 4 | London | A | Jackson | Reay | Roby | Gravely | 3:40.00 | Unverified: |
| 5 | Spring. ECA | A | Hayes | Hepp | Troutwine | Whetstone | 3:55.00 | Unverified: |
| 6 | Amanda-Clearcreek | A | Hinton | Williams | Gilman | Decker | NT | Unverified: |
| 7 | Mifflin | A | Thomas | Abdullahi | Sanders | Afriyie | NT |