2, 2, 4, 8
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Madison-Plains | A | NT | Unverified: | ||||
| 3 | Amanda-Clearcreek | A | Sowers | Kern | Leist | Berger | NT | Unverified: |
| 4 | London | A | Lickliter | Martinez | Powell | Colestock | 5:00.00 | Unverified: |
| 5 | Blanchester | A | Lewis | Griffith | Conley | Sears | NT | Unverified: |
| 6 | Spring. ECA | A | Spencer | Koch | Schlueter | Connor | NT | Unverified: |