| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Mifflin | A | Johnson | Duffy | Bellomy | Hoag | NT | |
| 3 | Amanda-Clearcreek | A | Sowers | Strickler | Ritchie | Conrad | NT | Unverified: |
| 4 | London | A | Lohrer | Weese | Weese | Carn | 4:00.00 | Unverified: |
| 5 | Spring. ECA | A | Glaze | Hamstra | Pinkleton | Witherow | 4:09.00 | Unverified: |
| 6 | Blanchester | A | Lewis | Sears | Abbott | Gilbert | 4:45.00 | Unverified: |