| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Loudonville | A | Caley | England | Harris | Finley | NT | |
| 3 | Bluffton | A | Shilling | Bowling | Johnstone | Smallwood | NT | |
| 4 | Centerburg | A | Indiciani | Forby | Waggoner | Sands | 53.60 | |
| 5 | Ridgedale | A | Richmond | Kramer | Turner | Cook | 56.60 | |
| 6 | Old Fort | A | Adelsperger | Miller | Howard | Patrick | 57.00 | |
| 7 | Johnstown | A | O'Hearn | Cherry | Clemens | Villegas | NT |