| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GREEN, Aryanna | Gilead Christian | NT | |
| 2 | AMBROSE, Sarah | Northmor | 1:00.00 | |
| 3 | TEDROW, Jordan | Lucas | 59.00 | |
| 4 | SACKMAN, Nalia | Northmor | 57.00 | |
| 5 | CASTLE, Layla | Northmor | 58.00 | |
| 6 | CASE, Leah | Lucas | 59.00 | |
| 7 | BONIN, Jillian | Gilead Christian | NT | |
| 8 | TURNER, Aleah | Genoa Christian | NT |