| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fredericktown MS | A | Hansen | Neighbarger | Payne | Stoerkel | 1:16.12 | Northmor MS Meet |
| 3 | Centerburg MS | A | Cody | Douglass | Kain | Kish | 1:05.00 | |
| 4 | Utica | A | Meier | Meek | Watson | Epps | 57.45 | Joe Kasper Invitational |
| 5 | Mount Gilead MS | A | Whipple | Whipple | Brannon | Scales | 1:02.68 | Triad MS Invitational |
| 6 | Lucas MS | A | 1:03.88 | Northmor MS Meet |