| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | New London | A | Speicher | Logan | Byron | Garret | 50.00 | |
| 3 | Loudonville | A | Sly | Carr | Gutierrez | Hershberger | 49.85 | West Holmes Knights' Invitationa |
| 4 | Lucas | A | Estep | Fairchild | Daley | Guegold | 47.61 | Madison Comprehensive Invitation |
| 5 | Danville | A | Hackman | Boeshart | Proper | Mickley | 47.74 | Freddie HS Tri |
| 6 | Smithville | A | Piatt | Kindy | McFarlin | Harmon | 48.1h | |
| 7 | Cardington-Lincoln | A | Adams | LEVERING | Nippert | dudgeon | 54.76 | 2024 Bucyrus Track Elks Invitati |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fredericktown | A | Anderson | Roush | Bellman | Estep | 47.52 | 2024 Bucyrus Track Elks Invitati |
| 2 | Rittman | A | Hawkins | Sonego | Dirling | Rastorfer | 46.42 | Bill Dodd Invitational |
| 3 | Cloverleaf | A | Hankins | Kurtz | Gray | Medina | 46.30 | Cloverleaf Early Bird Relays |
| 4 | Centerburg | A | Lemaster | Mead | Harris | Turske | 45.30 | |
| 5 | Hillsdale | A | Stack | Sloan | Young | McFadden | 45.66 | |
| 6 | East Knox | A | Baker | Lamneck | Madden | Clark | 46.16 | John McDonough Invitational |
| 7 | Mar. Highland | A | Hernandez | Fictner | Benedict | Newsome | 46.7h | |
| 8 | Northmor | A | Landin | Sippel | Amens | Stockdale | 47.52 |