| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clear Fork | A | Bostic | Eith | Gandee | Thrush | 1:53.24 | Madison Comprehensive Invitation |
| 4 | Northmor | A | Abrams | Kanagy | Keen | Ruhl | 2:03.30 | Freddie HS Tri |
| 5 | Fredericktown | A | Reiss | Foster | Broseus | Reed | 2:04.05 | Ridgedale Track Invite |