| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Fredericktown | A | Daniels | Anderson | Roush | Snow | 1:35.02 | Ridgedale Track Invite |
| 4 | Clear Fork | A | Damon | Kabuto | Bostic | Samuel | 1:35.22 | Highland, Clear Fork, Ontario, a |
| 5 | Centerburg | A | Clayton | Cornejo | Costa | Pizzuti | 1:45.00 |