| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 4 | AURAND, Jenna | Fredericktown | 1:19.76 | Freddie HS Quad |
| 5 | ERLSTEN, Lizzie | Northmor | 1:20.64 | Freddie HS Tri |
| 6 | GAYTON, Ava | Northmor | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | STATLER, Katie | Northmor | 1:14.05 | Cowen Invitational |
| 2 | SUTTON, Makayla | Northmor | 1:11.83 | Col. Crawford Grove/Keller Invit |
| 3 | MEADE, Gracie | Cardington-Lincoln | 1:09.44 | |
| 4 | HALLABRIN, Magi | Cardington-Lincoln | 1:06.61 | John McDonough Invitational |
| 5 | PELKEY, Eliana | Clear Fork | 1:06.67 | Madison Comprehensive Invitation |
| 6 | GANDEE, Estella | Clear Fork | 1:07.31 | Clear Fork HS 3-4-5 |
| 7 | STUPKA, Mary | Clear Fork | 1:12.71 | Madison Comprehensive Invitation |
| 8 | THRUSH, Paisley | Clear Fork | 1:12.91 | 2023 Marion Harding Quad |