| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HILL, Ahzirrea | St. Peter's | 1:02.57 | Mid Buckeye Conference Champions |
| 3 | WILSON, Mackenzie | East Knox | 57.60 | Jim Proper Invitational |
| 4 | GREEN, Aryanna | Gilead Christian | 56.79 | Jim Proper HS Invitational |
| 5 | FARRELL, Raegan | Utica HS | 56.81 | Lakewood Invitational |
| 6 | FICHTNER, Ava | Mar. Highland | 57.60 | |
| 7 | PETERSON, Brooklynn | Mans. Christian | 1:07.19 | Plymouth Memorial Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PRUETT, Abbie | Mar. Highland | 56.78 | |
| 2 | ALEXANDER, Jenny | Fredericktown | 54.88 | Freddie HS Quad |
| 3 | RICKETT, Cora | Mount Vernon | 53.54 | Hank Smith Invitational |
| 4 | RUTTER, Marissa | Mount Vernon | 47.08 | Heath Hank Smith Invitational |
| 5 | ARNOLD, Zoya | Col. Academy | 47.92 | OHSAA Division 2 District Girls |
| 6 | LOWRIE, Ella | Col. Academy | 48.65 | OHSAA Division 2 District Girls |
| 7 | YATES, Madison | Utica HS | 56.23 | Joe Kasper Invitational |
| 8 | PAYNE, Pink | Fredericktown | 56.41 | Cowen Invitational |